exRNAdisease

Entry Detail

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General Information

Database ID: exR0359188 RNA Name: AAK1 RNA Type: mRNA Chromosome: chr2 Starnd: - Coordinate: Start Site(bp): 69457997 End Site(bp): 69674349 External Links: ENSG00000115977

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACAP2 chr3 195274745 195443044 - ADD2 chr2 70607618 70768225 - BANP chr16 87949244 88077318 + CDC42 chr1 22025511 22101360 + CDK18 chr1 205504595 205532793 + CFLAR chr2 201116154 201176687 + CHPT1 chr12 101696947 101744140 + CNDP2 chr18 74495816 74523454 + CTNNA1 chr5 138610967 138935034 + GNL3 chr3 52681156 52694497 + HCN1 chr5 45254948 45696498 - HDLBP chr2 241227264 241317061 - HUWE1 chrX 53532096 53686728 - MT-ATP6 chrMT 8527 9207 + PAICS chr4 56435741 56464579 + PPP1CB chr2 28751640 28802940 + PRRC2B chr9 131373636 131500197 + PSMB2 chr1 35599541 35641526 - PTPRZ1 chr7 121873089 122062036 + SH2B3 chr12 111405923 111451623 + SLCO3A1 chr15 91853708 92172435 + SNRNP200 chr2 96274338 96321271 - SRP14 chr15 40035690 40039181 - SSH2 chr17 29625938 29930276 - TRIM65 chr17 75880335 75896951 - TRIO chr5 14143342 14532128 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC008764.6 chr19 16542746 16544814 + AC069544.1 chr10 14877688 14878686 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.