exRNAdisease

Entry Detail

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General Information

Database ID: exR0359267 RNA Name: ABT1 RNA Type: mRNA Chromosome: chr6 Starnd: + Coordinate: Start Site(bp): 26596953 End Site(bp): 26600739 External Links: ENSG00000146109

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand HSP90AB1 chr6 44246166 44253888 + RPS20 chr8 56067295 56074581 - SLC37A4 chr11 119024114 119030906 - PAFAH1B2 chr11 117144284 117176894 + RNASEH2C chr11 65714005 65720818 - CLTA chr9 36190856 36304781 + USF1 chr1 161039251 161045977 - POR chr7 75899200 75986855 + ENO1 chr1 8861000 8879190 - RPL22L1 chr3 170864875 170870208 - RAN chr12 130872037 130877678 + GSTP1 chr11 67583742 67586656 + PDLIM1 chr10 95237572 95291012 - MPDU1 chr17 7583529 7592789 + HSPD1 chr2 197486584 197516737 - THOC5 chr22 29505879 29555216 - PAK1IP1 chr6 10694972 10709782 + CHCHD10 chr22 23765834 23768443 - DHRS4L2 chr14 23969874 24006408 + GFOD1 chr6 13357830 13487662 - DNAJC14 chr12 55820960 55830824 - NFE2L1 chr17 48048329 48061545 + SFRP1 chr8 41261962 41309473 - B2M chr15 44711487 44718877 + BABAM1 chr19 17267376 17281249 + GTPBP1 chr22 38705742 38738299 + IL4R chr16 27313668 27364778 + HLA-DRA chr6 32439878 32445046 + MCFD2 chr2 46901870 46941855 - EEF1A1 chr6 73515750 73523797 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-873-5p chr9 28888925 28888945 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC008969.1 chr19 58002061 58011232 + AC020663.1 chr16 4730115 4752565 - MALAT1 chr11 65497688 65506516 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.