exRNAdisease

Entry Detail

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General Information

Database ID: exR0359270 RNA Name: ACAA1 RNA Type: mRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 38103129 End Site(bp): 38137242 External Links: ENSG00000060971

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand NDUFS8 chr11 68030617 68036644 + SYNGR2 chr17 78168558 78173527 + ATXN7L2 chr1 109483479 109492804 + EIF3B chr7 2354086 2380745 + MARCKSL1 chr1 32333839 32336233 - COTL1 chr16 84565596 84618078 - ZNF331 chr19 53519527 53580269 + RPS3 chr11 75399515 75422280 + ICMT chr1 6221193 6235972 - ZFHX3 chr16 72782885 73891871 - TPP1 chr11 6612768 6619448 - RPL37A chr2 216498825 216579180 + TIMP2 chr17 78852977 78925387 - ST6GAL1 chr3 186930502 187078553 + DCTN5 chr16 23641466 23677472 + PTOV1 chr19 49850735 49860744 + KCTD13 chr16 29905012 29926236 - TRAP1 chr16 3651639 3717553 - CEP295NL chr17 78870910 78903217 - RPL11 chr1 23691742 23696835 + LMNB1 chr5 126776623 126837020 + MRPS27 chr5 72219409 72320646 - DDX3Y chrY 12904108 12920478 + NPNT chr4 105894775 106004027 + MTRNR2L12 chr3 96617188 96618236 - LIN28A chr1 26410817 26429728 + MPRIP chr17 17042545 17217679 + HDAC1 chr1 32292083 32333635 + ZBTB40 chr1 22428838 22531157 + RPS4X chrX 72255679 72277248 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4449 chr4 52712720 52712741 + circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.