exRNAdisease

Entry Detail

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General Information

Database ID: exR0359271 RNA Name: ACAA2 RNA Type: mRNA Chromosome: chr18 Starnd: - Coordinate: Start Site(bp): 49782164 End Site(bp): 49813953 External Links: ENSG00000167315

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand DCAF5 chr14 69050881 69153150 - PSMA6 chr14 35278633 35317493 + RPL13 chr16 89560657 89566828 + GSPT1 chr16 11868128 11916082 - GABARAP chr17 7240008 7242449 - DNAJC1 chr10 21756548 22003769 - LRRN1 chr3 3799431 3849834 + SRSF7 chr2 38743599 38751494 - MAPKAP1 chr9 125437393 125707234 - CLDND1 chr3 98497912 98523066 - AC120057.2 chr17 7240427 7244635 - KDM5C chrX 53191321 53225422 - RAB6A chr11 73675638 73761137 - EXOC6B chr2 72175984 72826041 - CDC20 chr1 43358981 43363203 + SF3B6 chr2 24067586 24076373 - UBL5 chr19 9827892 9830115 + MTRNR2L12 chr3 96617188 96618236 - HACD4 chr9 20999509 21031640 - RAN chr12 130872037 130877678 + RAD17 chr5 69369293 69414801 + RPS6 chr9 19375715 19380236 - REEP5 chr5 112876385 112922289 - LRRFIP1 chr2 237627587 237813682 + VASP chr19 45506579 45526983 + CAPN1 chr11 65180566 65212006 + CKAP5 chr11 46743048 46846308 - NTS chr12 85874295 85882992 + ERN1 chr17 64039142 64130819 - PRDX6 chr1 173477330 173488815 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-492 chr12 94834427 94834449 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC026362.1 chr12 122975320 122982907 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.