exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359293 RNA Name: ACD RNA Type: mRNA Chromosome: chr16 Starnd: - Coordinate: Start Site(bp): 67657512 End Site(bp): 67660815 External Links: ENSG00000102977

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand DHX9 chr1 182839347 182887982 + DGCR2 chr22 19036282 19122454 - HSP90AB1 chr6 44246166 44253888 + AP002990.1 chr11 62559603 62591531 - ZNF23 chr16 71447597 71463095 - DUSP3 chr17 43766125 43778977 - EEF1G chr11 62559596 62574086 - SEC22C chr3 42547969 42601080 - SLC35B2 chr6 44254096 44257890 - PPM1A chr14 60245752 60299087 + CDK16 chrX 47217860 47229997 + MEPCE chr7 100428790 100434118 + FGFRL1 chr4 1009936 1026898 + NAA10 chrX 153929225 153935080 - MT-CO1 chrMT 5904 7445 + ATP6V1D chr14 67294371 67360265 - EFTUD2 chr17 44849948 44899445 - TFAM chr10 58385345 58399220 + RAF1 chr3 12583601 12664226 - MARCKSL1 chr1 32333839 32336233 - CNOT1 chr16 58519951 58629885 - FSTL3 chr19 676392 683392 + HNRNPD chr4 82352498 82374503 - PCBP2 chr12 53452102 53481162 + TSPAN9 chr12 3077355 3286564 + PSMA4 chr15 78540405 78552417 + GCN1 chr12 120127202 120194715 - COX14 chr12 50112082 50120457 + GNAS chr20 58839718 58911192 + RPS6KA1 chr1 26529761 26575030 + miRNA targets: NA circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.