exRNAdisease

Entry Detail

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General Information

Database ID: exR0359328 RNA Name: ACTA1 RNA Type: mRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 229431245 End Site(bp): 229434098 External Links: ENSG00000143632

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand PRMT2 chr21 46635595 46665124 + ACTR1B chr2 97655939 97664044 - SLC38A10 chr17 81244811 81295547 - SNRPB2 chr20 16729961 16742564 + AL136454.1 chr1 192716132 192716653 + TARDBP chr1 11012344 11026420 + EIF5A chr17 7306999 7312463 + ATP5PD chr17 75038863 75046985 - LIX1L chr1 145933423 145958017 - SLC25A3 chr12 98593591 98606379 + FTL chr19 48965309 48966879 + RPL28 chr19 55385345 55403250 + POTEJ chr2 130611481 130658091 + AGGF1 chr5 77029251 77065234 + MCM2 chr3 127598410 127622436 + ERGIC3 chr20 35542021 35557634 + HEXA chr15 72340919 72376476 - UBE4B chr1 10032832 10181239 + ACTC1 chr15 34790230 34795549 - RPL23 chr17 38847860 38853764 - SEC61G chr7 54752250 54759974 - MRPS27 chr5 72219409 72320646 - RPL11 chr1 23691742 23696835 + RNPEP chr1 201982372 202006147 + PFN1 chr17 4945652 4949061 - PPIA chr7 44796680 44824564 + RPL36 chr19 5674947 5691875 + POLR3C chr1 145824088 145844402 + TNIP1 chr5 151029945 151093577 - CDC42SE1 chr1 151050971 151070325 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4426 chr1 192716333 192716349 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL022311.1 chr22 37876148 37895563 + AL355472.2 chr1 234357006 234365828 + AL359924.1 chr1 237862175 237928321 + LINC00847 chr5 180830326 180839742 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.