exRNAdisease

Entry Detail

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General Information

Database ID: exR0359416 RNA Name: ADD1 RNA Type: mRNA Chromosome: chr4 Starnd: + Coordinate: Start Site(bp): 2843857 End Site(bp): 2930076 External Links: ENSG00000087274

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand HIRA chr22 19330698 19447450 - DDX17 chr22 38483438 38507660 - HNRNPAB chr5 178204533 178211163 + ENO1 chr1 8861000 8879190 - MFSD14A chr1 100038095 100083377 + RABGAP1L chr1 174159410 174995308 + MARCKSL1 chr1 32333839 32336233 - MTERF4 chr2 241072169 241102332 - EIF3L chr22 37848868 37889407 + CDC123 chr10 12195965 12250589 + RPS10 chr6 34417454 34426069 - PDHA2 chr4 95840093 95841464 + RPL9 chr4 39452521 39458949 - NPM1 chr5 171387116 171411137 + IFI44L chr1 78619922 78646145 + SAMD11 chr1 923928 944581 + B2M chr15 44711487 44718877 + POLE chr12 132623753 132687376 - HNRNPUL2 chr11 62712630 62727457 - ELOC chr8 73939169 73972287 - RGL2 chr6 33291654 33298942 - BIRC2 chr11 102347211 102378670 + HLA-A chr6 29941260 29945884 + RPL4 chr15 66498015 66524532 - RPS3 chr11 75399515 75422280 + LPCAT3 chr12 6976185 7018477 - CARHSP1 chr16 8852942 8869012 - CAPN15 chr16 527712 554636 + CLN6 chr15 68206992 68257211 - RPS27A chr2 55231903 55235853 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-184 chr15 79209840 79209861 + hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-193a-5p chr17 31560016 31560037 + hsa-miR-361-3p chrX 85903641 85903663 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand ACVR2B-AS1 chr3 38451027 38454820 - AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + AC009163.4 chr16 75458252 75460017 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.