exRNAdisease

Entry Detail

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General Information

Database ID: exR0359440 RNA Name: ADPGK RNA Type: mRNA Chromosome: chr15 Starnd: - Coordinate: Start Site(bp): 72751369 End Site(bp): 72785846 External Links: ENSG00000159322

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand EEF1A1 chr6 73515750 73523797 - B4GAT1 chr11 66345374 66347629 - MKNK2 chr19 2037465 2051244 - UBA2 chr19 34428352 34471251 + PGK1 chrX 77910739 78129295 + STAMBP chr2 73828916 73873659 + CCDC150 chr2 196639554 196763490 + SRSF1 chr17 58000919 58007346 - GTF3C2 chr2 27325849 27357034 - EIF3H chr8 116642130 116766925 - ACTB chr7 5527148 5563784 - GMCL1 chr2 69829660 69881384 + BCAR3 chr1 93561741 93847150 - UBE2V2 chr8 48008415 48064708 + MTHFD2 chr2 74198610 74217565 + MT-CO1 chrMT 5904 7445 + NOL8 chr9 92297358 92325636 - EFTUD2 chr17 44849948 44899445 - PPP1R14B chr11 64244479 64246943 - ZFP42 chr4 187995771 188005046 + ATG4B chr2 241637213 241673857 + NDUFB3 chr2 201071433 201085750 + BCKDK chr16 31106107 31112791 + PCBP2 chr12 53452102 53481162 + MORC4 chrX 106813871 107000212 - HMGB2 chr4 173331376 173334432 - SLC25A29 chr14 100291116 100306547 - TBCA chr5 77691166 77868780 - SACS chr13 23328826 23433740 - PASK chr2 241106099 241150264 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AC023509.1 chr12 53441741 53467528 + AC135050.1 chr16 31056460 31062803 + AL355488.1 chr1 110370154 110373003 + PCBP2-OT1 chr12 53464468 53465057 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.