exRNAdisease

Entry Detail

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General Information

Database ID: exR0359448 RNA Name: ADRA2C RNA Type: mRNA Chromosome: chr4 Starnd: + Coordinate: Start Site(bp): 3766348 End Site(bp): 3768526 External Links: ENSG00000184160

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010422.3 chr19 12643831 12648397 - AC093525.1 chr16 2513965 2527955 + AGPS chr2 177392757 177559299 + ATP6V0C chr16 2513952 2520218 + BRCA1 chr17 43044295 43170245 - EEF2 chr19 3976056 3985463 - EIF1 chr17 41688885 41692668 + EMID1 chr22 29205896 29259597 + MAGED1 chrX 51803007 51902357 + MARK2 chr11 63838928 63911019 + MFGE8 chr15 88898683 88913381 - NAP1L1 chr12 76036585 76084958 - PCBP1 chr2 70087477 70089203 + PKM chr15 72199029 72231822 - POLR3D chr8 22245133 22254601 + PRKD1 chr14 29576479 30191898 - PSIP1 chr9 15464066 15510995 - PTMA chr2 231706895 231713541 + RACK1 chr5 181236897 181248096 - RPL8 chr8 144789765 144792587 - SAMD11 chr1 923928 944581 + SH3BP4 chr2 234952017 235055714 + SNRPB chr20 2461634 2470853 - USO1 chr4 75724593 75814286 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC060780.1 chr17 43148368 43171037 - FOXD3-AS1 chr1 63320884 63324441 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.