exRNAdisease

Entry Detail

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General Information

Database ID: exR0359457 RNA Name: AEN RNA Type: mRNA Chromosome: chr15 Starnd: + Coordinate: Start Site(bp): 88621337 End Site(bp): 88632281 External Links: ENSG00000181026

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand HSP90AA1 chr14 102080738 102139699 - PTEN chr10 87863625 87971930 + MAPK9 chr5 180233143 180292099 - PABPC1 chr8 100685816 100722809 - ITM2C chr2 230864639 230879248 + KAT7 chr17 49788681 49835026 + HCCS chrX 11111301 11123086 + MESD chr15 80946289 80989828 - PPP1CB chr2 28751640 28802940 + PEBP1 chr12 118136124 118145584 + STARD7 chr2 96184859 96208825 - YBX1 chr1 42682418 42703805 + MYL6 chr12 56158161 56163496 + STRN4 chr19 46719511 46746994 - AC006538.2 chr19 2717769 2740048 - APH1A chr1 150265399 150269580 - JUNB chr19 12791486 12793315 + TSR2 chrX 54440404 54448032 + PLK1 chr16 23677656 23690367 + MATK chr19 3777970 3802129 - CSDE1 chr1 114716913 114758676 - FKBP8 chr19 18531751 18544077 - TOR1A chr9 129812942 129824244 - R3HDM2 chr12 57253762 57431005 - GBE1 chr3 81489703 81761645 - IVD chr15 40405485 40435947 + GPBP1 chr5 57173948 57264679 + ZNF202 chr11 123723914 123741675 - HSPA4 chr5 133052013 133106449 + MDK chr11 46380756 46383837 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-24-3p chr9 95086064 95086085 + hsa-miR-27a-3p chr19 13836447 13836467 - hsa-miR-101-3p chr1 65058442 65058462 - hsa-miR-27b-3p chr9 95085505 95085525 + hsa-miR-513a-5p chrX 147213538 147213555 - hsa-miR-455-5p chr9 114209449 114209470 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007952.4 chr17 19112000 19112636 - AC093827.4 chr4 86924630 86936202 - AD000090.1 chr19 35557956 35581954 + CCDC183-AS1 chr9 136803927 136808848 - LNCPRESS1 chr7 101299578 101301346 + NEAT1 chr11 65422774 65445540 + SNHG3 chr1 28505980 28510892 + XIST chrX 73820649 73852723 - ZFAS1 chr20 49278178 49299600 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.