exRNAdisease

Entry Detail

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General Information

Database ID: exR0359466 RNA Name: AFMID RNA Type: mRNA Chromosome: chr17 Starnd: + Coordinate: Start Site(bp): 78187317 End Site(bp): 78207701 External Links: ENSG00000183077

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ALDOA chr16 30064164 30070457 + C8orf88 chr8 90958471 90985238 - CCT7 chr2 73233420 73253021 + CFL1 chr11 65823022 65862026 - CNOT1 chr16 58519951 58629885 - CRYBG2 chr1 26321698 26360080 - CSRP2 chr12 76858709 76879023 - EEF1D chr8 143579697 143599541 - FLNA chrX 154348524 154374638 - GTPBP3 chr19 17334920 17342731 + HLA-DPB1 chr6 33075990 33089696 + HOXD9 chr2 176122719 176124937 + LMNB1 chr5 126776623 126837020 + LPCAT1 chr5 1456480 1523962 - MAP3K11 chr11 65597756 65615382 - MYO1C chr17 1464186 1492686 - NCBP3 chr17 3802158 3846246 - PCNP chr3 101574180 101594465 + PPIA chr7 44796680 44824564 + RANBP2 chr2 108719482 108785809 + RBM5 chr3 50088919 50119021 + SIPA1L1 chr14 71320449 71741229 + SRA1 chr5 140537340 140558252 - TLR7 chrX 12867072 12890361 + UHRF1BP1 chr6 34792015 34883138 + UQCRHL chr1 15807169 15809348 - USF2 chr19 35268962 35279821 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL021707.3 chr22 38734730 38738990 + RMRP chr9 35657751 35658018 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.