exRNAdisease

Entry Detail

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General Information

Database ID: exR0359537 RNA Name: AKAP10 RNA Type: mRNA Chromosome: chr17 Starnd: - Coordinate: Start Site(bp): 19904302 End Site(bp): 19978343 External Links: ENSG00000108599

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TCERG1 chr5 146447311 146511961 + EFNB2 chr13 106489745 106535662 - MCFD2 chr2 46901870 46941855 - ZNF850 chr19 36714383 36772825 - ATP5MC2 chr12 53632726 53677408 - PTPRF chr1 43525187 43623666 + NSMF chr9 137447573 137459334 - GUF1 chr4 44678420 44700928 + AMPD2 chr1 109616104 109632053 + MTG2 chr20 62183029 62203568 + AASDHPPT chr11 106075501 106098699 + PROM1 chr4 15963076 16084378 - RPL27 chr17 42998273 43002959 + MICALL1 chr22 37905657 37942822 + ZNF24 chr18 35332227 35345482 - CSNK1E chr22 38290691 38318084 - ICMT chr1 6221193 6235972 - ZFAND3 chr6 37819727 38154624 + SBNO2 chr19 1107637 1174268 - OGT chrX 71533104 71575892 + FOXK2 chr17 82519713 82644662 + CANX chr5 179678628 179731641 + VMP1 chr17 59707192 59842255 + CREBL2 chr12 12611827 12645108 + GIGYF2 chr2 232697299 232860575 + TNFSF9 chr19 6531026 6535924 + SOBP chr6 107490106 107661306 + AP2A2 chr11 924894 1012245 + SLC35F2 chr11 107790991 107928293 - AP002990.1 chr11 62559603 62591531 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-130a-3p chr11 57641252 57641273 + hsa-miR-130b-3p chr22 21653354 21653375 + hsa-miR-454-3p chr17 59137787 59137809 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079949.1 chr12 127142029 127146532 - AL645608.1 chr1 1011997 1013193 - TMEM147-AS1 chr19 35540738 35546029 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.