exRNAdisease

Entry Detail

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General Information

Database ID: exR0359569 RNA Name: ALAD RNA Type: mRNA Chromosome: chr9 Starnd: - Coordinate: Start Site(bp): 113386312 End Site(bp): 113401290 External Links: ENSG00000148218

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand SUSD2 chr22 24181487 24189106 + EIF4G2 chr11 10797050 10808940 - RTL6 chr22 44492583 44498233 - FAM32A chr19 16185380 16192046 + PKM chr15 72199029 72231822 - MRPL12 chr17 81703367 81707517 + NEK1 chr4 169392857 169612629 - NAPB chr20 23374519 23421519 - TAF6L chr11 62771357 62787342 + GAPDH chr12 6534512 6538374 + UBR5 chr8 102252273 102412759 - CHD4 chr12 6570082 6614524 - TIPIN chr15 66336191 66386746 - ZCCHC8 chr12 122471599 122501073 - HNRNPK chr9 83968083 83980616 - AC139530.2 chr17 81703371 81720539 + ARHGDIA chr17 81867721 81871378 - RPL4 chr15 66498015 66524532 - PFN1 chr17 4945652 4949061 - HLA-DRA chr6 32439878 32445046 + POU5F1 chr6 31164337 31180731 - YBX3 chr12 10699089 10723323 - RAB13 chr1 153981617 153986358 - RPL41 chr12 56116590 56117967 + HSPBP1 chr19 55262223 55280381 - PLEC chr8 143915147 143976734 - HELLS chr10 94501434 94613905 + PRC1 chr15 90966040 90995629 - CRMP1 chr4 5748084 5893086 - C17orf100 chr17 6651762 6693202 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC068831.7 chr15 91022766 91023200 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.