exRNAdisease

Entry Detail

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General Information

Database ID: exR0361286 RNA Name: CFL1 RNA Type: mRNA Chromosome: chr11 Starnd: - Coordinate: Start Site(bp): 65823022 End Site(bp): 65862026 External Links: ENSG00000172757

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AKAP8 chr19 15353385 15379798 - AC004922.1 chr7 99325879 99394653 + AL133352.1 chr10 100505628 100529881 - AHCYL2 chr7 129225023 129430211 + AC010422.6 chr19 12525720 12580975 - AHCY chr20 34280268 34311802 - AFDN chr6 167826922 167972023 + ALDH6A1 chr14 74056847 74084492 - AC073082.1 chr2 48632291 48755724 - AEN chr15 88621337 88632281 + ACTG1 chr17 81509971 81523847 - AFG3L2 chr18 12328944 12377227 - AASDHPPT chr11 106075501 106098699 + AHNAK2 chr14 104937244 104978374 - AKT2 chr19 40230317 40285536 - ACTB chr7 5527148 5563784 - ADPRH chr3 119579268 119589945 + AC092647.5 chr7 55887277 55955239 + ACBD3 chr1 226144679 226186741 - ABCB7 chrX 75051048 75156732 - ACTA1 chr1 229431245 229434098 - ABI1 chr10 26746593 26861087 - AC011462.1 chr19 41350853 41425001 + AKR1C1 chr10 4963253 4983283 + ACAT2 chr6 159762045 159779112 + AC010463.1 chr19 17267418 17282966 + AC010422.3 chr19 12643831 12648397 - AKAP12 chr6 151239967 151358559 + AKR1B1 chr7 134442356 134459284 - AC009133.6 chr16 29812261 29820092 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-155-5p chr21 25573983 25574006 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002470.2 chr22 20979462 20998121 + AC004687.1 chr17 58330884 58332508 - AC007952.4 chr17 19112000 19112636 - AC026362.1 chr12 122975320 122982907 + AC132217.1 chr11 2129121 2129964 - AC144548.1 chr12 110387463 110445548 - ADARB2-AS1 chr10 1526637 1556984 + AFDN-DT chr6 167822103 167826812 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.