exRNAdisease

Entry Detail

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General Information

Database ID: exR0362563 RNA Name: EEF1D RNA Type: mRNA Chromosome: chr8 Starnd: - Coordinate: Start Site(bp): 143579697 End Site(bp): 143599541 External Links: ENSG00000104529

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAMP chr2 218264123 218270257 - ABCA2 chr9 137007234 137028922 - ABCC5 chr3 183919934 184017939 - ABHD10 chr3 111979010 111993368 + AC012184.2 chr16 70299194 70372582 + AC074143.1 chr16 67163385 67165815 + AC090004.1 chr3 14135217 14148252 + AC092143.1 chr16 89919165 89936092 + AC092647.5 chr7 55887277 55955239 + AC093525.2 chr16 2496032 2520218 + AC244197.3 chrX 149477104 149540926 - AC245033.1 chr15 82536788 82573194 - ACLY chr17 41866917 41930542 - ACTA1 chr1 229431245 229434098 - ACTB chr7 5527148 5563784 - ACTC1 chr15 34790230 34795549 - ACTG1 chr17 81509971 81523847 - ACTR3 chr2 113890063 113962596 + ADGRE5 chr19 14380501 14408725 + AFMID chr17 78187317 78207701 + AHCYL2 chr7 129225023 129430211 + AL713999.1 chr1 155169409 155187272 - ALDH9A1 chr1 165662216 165698863 - ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4517 chr16 28958587 28958611 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC067930.1 chr8 143579636 143580670 + AC069281.2 chr7 100572232 100578700 - AC245033.4 chr15 82533175 82540008 - AD000090.1 chr19 35557956 35581954 + AL132780.1 chr14 22929607 22956374 + AL133166.1 chr14 29210986 29392048 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.