exRNAdisease

Entry Detail

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General Information

Database ID: exR0362939 RNA Name: EZR RNA Type: mRNA Chromosome: chr6 Starnd: - Coordinate: Start Site(bp): 158765741 End Site(bp): 158819368 External Links: ENSG00000092820

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABRACL chr6 139028745 139043302 + AC004922.1 chr7 99325879 99394653 + AC010132.3 chr7 42909273 42932174 - AC092143.1 chr16 89919165 89936092 + AC245033.1 chr15 82536788 82573194 - ACAT1 chr11 108116695 108147603 + ACKR3 chr2 236567787 236582354 + ACTB chr7 5527148 5563784 - ACTR1A chr10 102461881 102502712 - ACTR3 chr2 113890063 113962596 + ADH5 chr4 99070978 99088801 - ADIPOR1 chr1 202940826 202958572 - ADSL chr22 40346500 40390463 + AFDN chr6 167826922 167972023 + AGBL5 chr2 27042364 27070622 + AGPAT2 chr9 136673143 136687457 - AL022238.4 chr22 40346529 40410054 + AL136295.4 chr14 24180395 24190416 - AL365205.1 chr6 41780349 41790141 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-92a-3p chrX 134169544 134169565 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010733.2 chr2 60925909 60931610 + AC023509.1 chr12 53441741 53467528 + AC106864.1 chr4 112693047 112706810 - AC110619.1 chr2 240449315 240456714 - AC126365.1 chr17 20788071 20789584 + AC132217.1 chr11 2129121 2129964 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.