exRNAdisease

Entry Detail

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General Information

Database ID: exR0363479 RNA Name: FUS RNA Type: mRNA Chromosome: chr16 Starnd: + Coordinate: Start Site(bp): 31180110 End Site(bp): 31194871 External Links: ENSG00000089280

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAGAB chr15 67200667 67255195 - ACTN4 chr19 38647649 38731589 + ADCY6 chr12 48766194 48789037 - AC091167.2 chr15 90249556 90272208 + AC007192.1 chr19 18153158 18178117 + AHCY chr20 34280268 34311802 - AAMP chr2 218264123 218270257 - AC010422.3 chr19 12643831 12648397 - ABRACL chr6 139028745 139043302 + ACTR2 chr2 65227753 65271253 + AC093525.1 chr16 2513965 2527955 + AFDN chr6 167826922 167972023 + AL365205.1 chr6 41780349 41790141 + AEBP2 chr12 19404045 19720801 + AC093525.2 chr16 2496032 2520218 + ACTR5 chr20 38748460 38772520 + ACTB chr7 5527148 5563784 - AL359736.1 chr13 23979700 24321031 + AC005832.4 chr12 4604944 4662643 + AKT1S1 chr19 49869033 49878459 - AC092143.1 chr16 89919165 89936092 + ALDH4A1 chr1 18871430 18902724 - ADGRL2 chr1 81306160 81992436 + AC004922.1 chr7 99325879 99394653 + ALDOA chr16 30064164 30070457 + AGTRAP chr1 11736084 11754802 + AC008878.3 chr19 7382834 7470241 + AC138811.2 chr16 18788063 18801519 - ACTG1 chr17 81509971 81523847 - AKR7A2 chr1 19303965 19312146 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-221-3p chrX 45746180 45746202 - hsa-miR-4508 chr15 23562107 23562123 - hsa-miR-4521 chr17 8186948 8186969 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005726.3 chr17 28573117 28574243 + AC020928.2 chr19 36773712 36775908 - AC023509.1 chr12 53441741 53467528 + AC079781.5 chr7 97851688 97972985 - AC096992.2 chr3 136837338 136839021 - AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + AL031282.2 chr1 1702736 1737688 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.