exRNAdisease

Entry Detail

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General Information

Database ID: exR0363770 RNA Name: GNAS RNA Type: mRNA Chromosome: chr20 Starnd: + Coordinate: Start Site(bp): 58839718 End Site(bp): 58911192 External Links: ENSG00000087460

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AADAT chr4 170060222 170091699 - AL133352.1 chr10 100505628 100529881 - AC138811.2 chr16 18788063 18801519 - ACLY chr17 41866917 41930542 - AC004922.1 chr7 99325879 99394653 + ABRACL chr6 139028745 139043302 + AC012254.2 chr18 47108188 47176345 - AGFG1 chr2 227472152 227561214 + AKAP17A chrX 1591604 1602520 + AC005258.1 chr19 2269525 2341172 + ADCY2 chr5 7396138 7830081 + AIG1 chr6 143060496 143341058 + ADGRL2 chr1 81306160 81992436 + ACKR3 chr2 236567787 236582354 + ABI1 chr10 26746593 26861087 - ADA chr20 44619522 44652233 - AKT1 chr14 104769349 104795751 - ACTN4 chr19 38647649 38731589 + AGO3 chr1 35930718 36072500 + AC139530.2 chr17 81703371 81720539 + AC140504.1 chr16 15395754 15515348 + AC245033.1 chr15 82536788 82573194 - AHNAK chr11 62433542 62556235 - AC087289.3 chr17 75898645 75930129 - ADCY1 chr7 45574140 45723116 + ABHD17C chr15 80679684 80755621 + AL136531.2 chr20 1317571 1393096 - ALDH9A1 chr1 165662216 165698863 - AC092647.5 chr7 55887277 55955239 + AC010422.3 chr19 12643831 12648397 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-20a-5p chr13 91351072 91351094 + hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-146a-5p chr5 160485372 160485393 + hsa-miR-1181 chr19 10403505 10403525 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007637.1 chr12 109111218 109125594 + AC007952.4 chr17 19112000 19112636 - AC010442.1 chr5 466124 473098 - AC010642.2 chr19 58309727 58327248 - AC011447.3 chr19 20122569 20321305 - AC016876.2 chr17 7581964 7584086 - AC023509.1 chr12 53441741 53467528 + AC040162.3 chr16 67882461 67886367 + AC090587.2 chr11 3854612 3855399 + AC106864.1 chr4 112693047 112706810 - AC124068.2 chr15 89335053 89336161 + AC132217.1 chr11 2129121 2129964 - AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.