exRNAdisease

Entry Detail

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General Information

Database ID: exR0364218 RNA Name: HLA-B RNA Type: mRNA Chromosome: chr6 Starnd: - Coordinate: Start Site(bp): 31269491 End Site(bp): 31357188 External Links: ENSG00000234745

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACTA2 chr10 88935074 88991339 - ACTG1 chr17 81509971 81523847 - ADPRHL2 chr1 36088892 36093932 + AK4 chr1 65147549 65232145 + AC092647.5 chr7 55887277 55955239 + ADM chr11 10305073 10307397 + AHCY chr20 34280268 34311802 - ACTB chr7 5527148 5563784 - ABCC10 chr6 43427366 43450427 + ACOT7 chr1 6264269 6394391 - ADAR chr1 154582057 154628013 - AGTRAP chr1 11736084 11754802 + ADI1 chr2 3497366 3519531 - AK2 chr1 33007940 33080996 - ABLIM1 chr10 114431113 114768061 - AC069503.2 chr12 121888809 121921470 + AL109827.1 chr20 35632340 35674544 - AGAP3 chr7 151085831 151144436 + AC011462.1 chr19 41350853 41425001 + AC135050.2 chr16 31083439 31094956 - AC046185.1 chr17 63702845 63752097 - ADRM1 chr20 62302093 62308862 + AC010422.3 chr19 12643831 12648397 - AC005258.1 chr19 2269525 2341172 + ADGRG2 chrX 18989307 19122637 - ACTR3 chr2 113890063 113962596 + ADCY1 chr7 45574140 45723116 + ACTA1 chr1 229431245 229434098 - ACADSB chr10 123008979 123058290 + AICDA chr12 8602170 8612867 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-155-5p chr21 25573983 25574006 + hsa-miR-6891-5p chr6 31355297 31355315 - hsa-miR-6516-5p chr17 77089428 77089449 + hsa-miR-6516-3p chr17 77089469 77089490 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002470.2 chr22 20979462 20998121 + AC006942.1 chr19 49808933 49809738 + AC019205.1 chr6 73263212 73301789 + AC023509.1 chr12 53441741 53467528 + AC067852.2 chr17 42552436 42554748 - AC091564.7 chr11 6610883 6616594 - AC093157.1 chr1 101025844 101090513 + AC134043.2 chr5 159209921 159248796 + AC139530.1 chr17 81701324 81703300 - AC147651.4 chr7 603185 608482 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.