exRNAdisease

Entry Detail

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General Information

Database ID: exR0364285 RNA Name: HNRNPU RNA Type: mRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 244840638 End Site(bp): 244864560 External Links: ENSG00000153187

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCC4 chr13 95019835 95301475 - AC004922.1 chr7 99325879 99394653 + AC010422.3 chr19 12643831 12648397 - AC012184.2 chr16 70299194 70372582 + AC116366.3 chr5 132410832 132646079 + AC131160.1 chr3 183815568 183884889 - AC138811.2 chr16 18788063 18801519 - AC140504.1 chr16 15395754 15515348 + ACLY chr17 41866917 41930542 - ACSL3 chr2 222860942 222944639 + ACTA1 chr1 229431245 229434098 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR1A chr10 102461881 102502712 - ADARB1 chr21 45073853 45226560 + ADD1 chr4 2843857 2930076 + ADGRV1 chr5 90529344 91164437 + ADPRHL2 chr1 36088892 36093932 + AFTPH chr2 64524305 64593005 + AGO2 chr8 140520156 140635633 - AGO4 chr1 35808016 35857890 + AGRN chr1 1020120 1056118 + AHCY chr20 34280268 34311802 - AIDA chr1 222668013 222713210 - AKAP8L chr19 15380050 15419141 - AKNA chr9 114334156 114394405 - AL136531.2 chr20 1317571 1393096 - AL138752.2 chr9 37588413 38068687 - ALDH1B1 chr9 38392702 38398661 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1244 chr2 231713368 231713393 + hsa-miR-3654 chr7 133034860 133034878 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010542.4 chr16 66549280 66551189 + AC016876.2 chr17 7581964 7584086 - AC124068.2 chr15 89335053 89336161 + AC132217.1 chr11 2129121 2129964 - AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.