exRNAdisease

Entry Detail

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General Information

Database ID: exR0365350 RNA Name: LIN28A RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 26410817 End Site(bp): 26429728 External Links: ENSG00000131914

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AHCYL2 chr7 129225023 129430211 + AGAP1 chr2 235494043 236131800 + ACKR3 chr2 236567787 236582354 + AC131160.1 chr3 183815568 183884889 - ACTR3B chr7 152759749 152855378 + AC006064.6 chr12 6556886 6607367 - AC027644.4 chr7 66628958 66811189 + AC008575.1 chr5 112827213 112867582 + AASS chr7 122073549 122144255 - AC073508.2 chr17 40627356 40665141 - ACOT2 chr14 73567620 73575658 + ADAR chr1 154582057 154628013 - AC004832.3 chr22 30409255 30428990 + ABCE1 chr4 145098288 145129524 + AKAP8L chr19 15380050 15419141 - AKR7A2 chr1 19303965 19312146 - ADCY2 chr5 7396138 7830081 + AL139300.1 chr14 103562962 103685924 + ALDH4A1 chr1 18871430 18902724 - AK7 chr14 96392128 96489427 + ABI2 chr2 203328239 203447728 + AC007192.1 chr19 18153158 18178117 + AL138752.2 chr9 37588413 38068687 - AC118549.1 chr1 77562416 77683419 - ACAA1 chr3 38103129 38137242 - ADGRL1 chr19 14147743 14206187 - ADD3 chr10 109996368 110135565 + AK2 chr1 33007940 33080996 - ABHD13 chr13 108218392 108234243 + ACTB chr7 5527148 5563784 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-3654 chr7 133034860 133034878 - hsa-miR-664b-5p chrX 154768596 154768619 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009446.1 chr8 71675300 71702786 + AC012615.1 chr19 1874871 1876169 - AC016747.4 chr2 61115787 61164825 + AC016876.2 chr17 7581964 7584086 - AC106864.1 chr4 112693047 112706810 - AC114284.1 chr5 120781218 120790778 + AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + AL137782.1 chr13 75549773 75807120 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.