exRNAdisease

Entry Detail

---



General Information

Database ID: exR0365738 RNA Name: MARCKSL1 RNA Type: mRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 32333839 End Site(bp): 32336233 External Links: ENSG00000175130

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCD4 chr14 74285269 74303055 - ABCF3 chr3 184186023 184194012 + AC005832.4 chr12 4604944 4662643 + AC008581.2 chr5 77030902 77152155 + AC008982.1 chr19 38817471 38840178 - AC010422.3 chr19 12643831 12648397 - AC011448.1 chr19 19516227 19536076 + AC012254.2 chr18 47108188 47176345 - AC091959.3 chr5 146203550 146339251 + ACAA1 chr3 38103129 38137242 - ACACA chr17 37084992 37406836 - ACD chr16 67657512 67660815 - ACIN1 chr14 23058564 23095614 - ACO2 chr22 41469117 41528989 + ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR3 chr2 113890063 113962596 + ADAM17 chr2 9488486 9556732 - ADAMTS13 chr9 133414358 133459402 + ADAMTS2 chr5 179110853 179345461 - ADD1 chr4 2843857 2930076 + ADIPOR1 chr1 202940826 202958572 - AGGF1 chr5 77029251 77065234 + AGL chr1 99850361 99924023 + AHDC1 chr1 27534035 27604431 - AIF1L chr9 131096476 131123152 + AKT1 chr14 104769349 104795751 - AL136454.1 chr1 192716132 192716653 + AL365205.1 chr6 41780349 41790141 + AL807752.6 chr9 137031241 137040436 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-let-7a-5p chr9 94175962 94175983 + hsa-let-7b-5p chr22 46113691 46113712 + hsa-let-7g-5p chr3 52268336 52268357 - hsa-miR-541-3p chr14 101064548 101064569 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC026362.1 chr12 122975320 122982907 + AC060780.1 chr17 43148368 43171037 - AC067852.2 chr17 42552436 42554748 - AC069281.2 chr7 100572232 100578700 - AC097359.2 chr3 37241789 37244177 - AC104257.1 chr8 131308545 131317632 + AC112229.4 chr2 110386411 110433673 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.