exRNAdisease

Entry Detail

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General Information

Database ID: exR0365991 RNA Name: MKNK2 RNA Type: mRNA Chromosome: chr19 Starnd: - Coordinate: Start Site(bp): 2037465 End Site(bp): 2051244 External Links: ENSG00000099875

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCD1 chrX 153724856 153744755 + ABCF2 chr7 151207837 151227166 - ABHD17A chr19 1876810 1885547 - AC010422.3 chr19 12643831 12648397 - AC010531.1 chr16 87302876 87334273 - ACADVL chr17 7217125 7225266 + ACAT2 chr6 159762045 159779112 + ACSL4 chrX 109624244 109733403 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ADGRE5 chr19 14380501 14408725 + ADPGK chr15 72751369 72785846 - AHCY chr20 34280268 34311802 - AIP chr11 67483026 67491103 + AK2 chr1 33007940 33080996 - AK4 chr1 65147549 65232145 + AL133352.1 chr10 100505628 100529881 - AL590764.2 chrX 71103987 71111575 - AL928654.3 chr14 105487199 105492267 + ALDH2 chr12 111766887 111817532 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1321 chrX 85835832 85835849 + hsa-miR-3654 chr7 133034860 133034878 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC008738.7 chr19 33207129 33207639 + AC010997.3 chr10 75279726 75401246 - AC016876.2 chr17 7581964 7584086 - AC245884.11 chr19 54308915 54337168 - AD000090.1 chr19 35557956 35581954 + AL049796.1 chr1 93847174 93848939 + AL138756.1 chr9 112028570 112039143 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.