exRNAdisease

Entry Detail

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General Information

Database ID: exR0366354 RNA Name: MYH9 RNA Type: mRNA Chromosome: chr22 Starnd: - Coordinate: Start Site(bp): 36281277 End Site(bp): 36388067 External Links: ENSG00000100345

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABI2 chr2 203328239 203447728 + ABLIM1 chr10 114431113 114768061 - AC010616.1 chr19 41956879 41994232 - AC018523.2 chr11 14493783 14520344 - ACAD10 chr12 111686056 111757107 + ACTA1 chr1 229431245 229434098 - ACTB chr7 5527148 5563784 - ACTN4 chr19 38647649 38731589 + ACTR1A chr10 102461881 102502712 - AD000671.1 chr19 35745678 35754519 + ADAM19 chr5 157395534 157575775 - ADCY6 chr12 48766194 48789037 - ADGRE1 chr19 6887566 6940459 + AK4 chr1 65147549 65232145 + ALDH16A1 chr19 49453225 49471050 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-25-3p chr7 100093571 100093592 - hsa-miR-214-3p chr1 172138816 172138837 - hsa-miR-27b-3p chr9 95085505 95085525 + hsa-miR-185-5p chr22 20033153 20033174 + hsa-miR-485-5p chr14 101055427 101055448 + hsa-miR-650 chr22 22822791 22822811 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-3609 chr7 98881700 98881723 + hsa-miR-6819-3p chr22 36286847 36286867 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005899.4 chr17 32328441 32329395 + AC016876.2 chr17 7581964 7584086 - AC019080.1 chr2 177283508 177392691 - AC026362.1 chr12 122975320 122982907 + AC051619.7 chr15 45200325 45200632 - AC106864.1 chr4 112693047 112706810 - AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.