exRNAdisease

Entry Detail

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General Information

Database ID: exR0366456 RNA Name: NAP1L1 RNA Type: mRNA Chromosome: chr12 Starnd: - Coordinate: Start Site(bp): 76036585 End Site(bp): 76084958 External Links: ENSG00000187109

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AGO4 chr1 35808016 35857890 + AC104109.3 chr5 134167170 134226071 - AC007040.2 chr2 70939318 70995336 - AFDN chr6 167826922 167972023 + AKR1E2 chr10 4786629 4848062 + ABHD8 chr19 17292131 17310236 - AC118549.1 chr1 77562416 77683419 - AL139353.1 chr14 31334312 31457441 - ALDH2 chr12 111766887 111817532 + ACTR3 chr2 113890063 113962596 + AIF1L chr9 131096476 131123152 + AGPAT5 chr8 6708642 6761503 + AC107871.1 chr15 68184032 68229718 - ACOT7 chr1 6264269 6394391 - ACTB chr7 5527148 5563784 - ADCY2 chr5 7396138 7830081 + AFTPH chr2 64524305 64593005 + AC007192.1 chr19 18153158 18178117 + AC008764.1 chr19 16479067 16628204 - AC120057.2 chr17 7240427 7244635 - AC139530.2 chr17 81703371 81720539 + ACKR3 chr2 236567787 236582354 + AL133352.1 chr10 100505628 100529881 - AHCYL1 chr1 109984765 110023742 + AC138811.2 chr16 18788063 18801519 - AGAP5 chr10 73674285 73698159 - ALAD chr9 113386312 113401290 - AC027796.3 chr17 3585149 3636249 - ADNP2 chr18 80109262 80147523 + AL669918.1 chr6 32813767 32838822 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007161.3 chr7 7640752 8004053 + AC007686.3 chr14 77028086 77031572 + AC020928.2 chr19 36773712 36775908 - AC022400.3 chr10 73674295 73730466 - AC022762.2 chr11 6201901 6203253 - AC084824.5 chr12 32736930 32737660 - AC116407.1 chr17 32141226 32143135 - AC125257.1 chr17 41848518 41851447 - AC144548.1 chr12 110387463 110445548 - AD000090.1 chr19 35557956 35581954 + AF131215.5 chr8 11107788 11109726 - AL022316.1 chr22 42438023 42446195 + AL121992.1 chr1 15586136 15603626 - AL133346.1 chr6 131950946 132077393 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.