exRNAdisease

Entry Detail

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General Information

Database ID: exR0367277 RNA Name: PAICS RNA Type: mRNA Chromosome: chr4 Starnd: + Coordinate: Start Site(bp): 56435741 End Site(bp): 56464579 External Links: ENSG00000128050

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAK1 chr2 69457997 69674349 - AAR2 chr20 36236459 36270918 + ABHD18 chr4 127965306 128039927 + AC120057.2 chr17 7240427 7244635 - AC135050.2 chr16 31083439 31094956 - AC245033.1 chr15 82536788 82573194 - ACOT7 chr1 6264269 6394391 - ACSS1 chr20 25006230 25058980 - ACTR8 chr3 53867066 53882152 - ADA2 chr22 17178790 17258235 - ADD1 chr4 2843857 2930076 + ADI1 chr2 3497366 3519531 - AEN chr15 88621337 88632281 + AGO3 chr1 35930718 36072500 + AGPAT3 chr21 43865223 43987592 + AHCYL1 chr1 109984765 110023742 + AIMP2 chr7 6009255 6023834 + AK2 chr1 33007940 33080996 - ALDH9A1 chr1 165662216 165698863 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005726.3 chr17 28573117 28574243 + AC016044.1 chr15 52800168 52805972 - AC020917.2 chr19 16440946 16443584 + AC022140.1 chr5 25404733 25445925 - AC093827.4 chr4 86924630 86936202 - AC105383.1 chr4 133075311 133149147 - AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.