exRNAdisease

Entry Detail

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General Information

Database ID: exR0367579 RNA Name: PGAM1 RNA Type: mRNA Chromosome: chr10 Starnd: + Coordinate: Start Site(bp): 97426191 End Site(bp): 97433444 External Links: ENSG00000171314

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AASDHPPT chr11 106075501 106098699 + ABCB6 chr2 219209772 219218994 - ABHD17C chr15 80679684 80755621 + ABI2 chr2 203328239 203447728 + ABL2 chr1 179099330 179229684 - AC007846.2 chr17 5772926 6080792 + AC073082.1 chr2 48632291 48755724 - AC091959.3 chr5 146203550 146339251 + AC110275.1 chr8 43018424 43077334 + AC139530.2 chr17 81703371 81720539 + AC245033.1 chr15 82536788 82573194 - ACACA chr17 37084992 37406836 - ACAT2 chr6 159762045 159779112 + ACBD7 chr10 15075475 15088776 - ACTB chr7 5527148 5563784 - ACTR1A chr10 102461881 102502712 - ACTR1B chr2 97655939 97664044 - ADA chr20 44619522 44652233 - ADAR chr1 154582057 154628013 - ADD2 chr2 70607618 70768225 - AFAP1 chr4 7758714 7939926 - AGBL5 chr2 27042364 27070622 + AKT2 chr19 40230317 40285536 - AL024498.2 chr6 10747794 10930423 + AL157392.5 chr10 13610047 13655929 + ALDH18A1 chr10 95605941 95656711 - ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-423-5p chr17 30117095 30117117 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009022.1 chr16 69976297 70065948 - AC026461.1 chr16 56682470 56687807 + AC245033.4 chr15 82533175 82540008 - AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.