exRNAdisease

Entry Detail

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General Information

Database ID: exR0367742 RNA Name: PKM RNA Type: mRNA Chromosome: chr15 Starnd: - Coordinate: Start Site(bp): 72199029 End Site(bp): 72231822 External Links: ENSG00000067225

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AASDHPPT chr11 106075501 106098699 + AC027796.3 chr17 3585149 3636249 - AC091167.2 chr15 90249556 90272208 + ACACB chr12 109116595 109268226 + AL136454.1 chr1 192716132 192716653 + AC093525.2 chr16 2496032 2520218 + AL121594.1 chr14 35122549 35317474 + AHCY chr20 34280268 34311802 - ACADM chr1 75724347 75787575 + ADA chr20 44619522 44652233 - AKT1S1 chr19 49869033 49878459 - ADAR chr1 154582057 154628013 - ACTG2 chr2 73892314 73919865 + AASS chr7 122073549 122144255 - AL121753.1 chr20 35267885 35280043 - ADAM22 chr7 87934143 88202889 + AHNAK chr11 62433542 62556235 - ADPGK chr15 72751369 72785846 - AC011448.1 chr19 19516227 19536076 + ACO2 chr22 41469117 41528989 + AC011462.1 chr19 41350853 41425001 + AC093323.1 chr4 6663396 6676755 + AD000671.2 chr19 35739252 35745432 - ACTG1 chr17 81509971 81523847 - ACTC1 chr15 34790230 34795549 - ACAT2 chr6 159762045 159779112 + AL133352.1 chr10 100505628 100529881 - AIP chr11 67483026 67491103 + AC010422.3 chr19 12643831 12648397 - AF241726.2 chrX 37349330 38687674 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-367-3p chr4 112647877 112647898 - hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-541-3p chr14 101064548 101064569 + hsa-miR-1286 chr22 20249145 20249165 - hsa-miR-3610 chr8 116874740 116874759 - hsa-miR-5587-5p chr16 535316 535333 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005899.4 chr17 32328441 32329395 + AC010327.5 chr19 55216660 55221616 + AC016876.2 chr17 7581964 7584086 - AC020663.1 chr16 4730115 4752565 - AC020928.1 chr19 36773112 36777078 + AC024940.6 chr12 31280422 31280895 - AC068205.2 chr11 43578889 43840030 + AC079781.5 chr7 97851688 97972985 - AC093827.4 chr4 86924630 86936202 - AC100861.1 chr8 23224471 23230926 + AC104461.1 chr1 200333193 200478669 + AC106875.1 chr2 11681460 11683328 + AC109460.3 chr16 28974804 28990775 + AC132217.1 chr11 2129121 2129964 - AC135048.1 chr16 30948386 30956511 + AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + AL031282.2 chr1 1702736 1737688 - AL049796.1 chr1 93847174 93848939 + AL110115.2 chr20 31580890 31581214 + AL121992.1 chr1 15586136 15603626 - AL137058.2 chr13 52600042 52642542 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.