exRNAdisease

Entry Detail

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General Information

Database ID: exR0368088 RNA Name: PPP2R1A RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 52190048 End Site(bp): 52229518 External Links: ENSG00000105568

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCF1 chr6 30571393 30597179 + ABL1 chr9 130713016 130887675 + AC006064.6 chr12 6556886 6607367 - AC008750.8 chr19 51350373 51368099 - AC010422.3 chr19 12643831 12648397 - AC245033.1 chr15 82536788 82573194 - ACADS chr12 120725774 120740008 + ACLY chr17 41866917 41930542 - ACSS1 chr20 25006230 25058980 - ACTG1 chr17 81509971 81523847 - ADAM10 chr15 58588809 58749791 - ADAMTS13 chr9 133414358 133459402 + ADAMTSL4 chr1 150549369 150560937 + ADD3 chr10 109996368 110135565 + ADH5 chr4 99070978 99088801 - AEN chr15 88621337 88632281 + AKIP1 chr11 8911139 8920084 + AKIRIN1 chr1 38991276 39006059 + AL136295.4 chr14 24180395 24190416 - ALDH18A1 chr10 95605941 95656711 - ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-625-5p chr14 65471116 65471136 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007780.1 chr17 68413623 68524949 + AC008764.6 chr19 16542746 16544814 + AC016876.2 chr17 7581964 7584086 - AC019069.1 chr2 74832655 74833987 - AC020663.1 chr16 4730115 4752565 - AC026461.1 chr16 56682470 56687807 + AC079781.5 chr7 97851688 97972985 - AC132217.1 chr11 2129121 2129964 - AC245033.4 chr15 82533175 82540008 - AL021408.1 chr6 132085084 132099279 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.