exRNAdisease

Entry Detail

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General Information

Database ID: exR0368411 RNA Name: PTMS RNA Type: mRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 6765516 End Site(bp): 6770952 External Links: ENSG00000159335

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AARSD1 chr17 42950526 42964498 - ABCG2 chr4 88090150 88231628 - ABHD16A chr6 31686955 31703356 - ABHD8 chr19 17292131 17310236 - ABI1 chr10 26746593 26861087 - AC010422.3 chr19 12643831 12648397 - AC011462.1 chr19 41350853 41425001 + AC073508.2 chr17 40627356 40665141 - AC073896.1 chr12 56285916 56316059 - AC087289.3 chr17 75898645 75930129 - AC131160.1 chr3 183815568 183884889 - AC245033.1 chr15 82536788 82573194 - ACIN1 chr14 23058564 23095614 - ACO2 chr22 41469117 41528989 + ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTN1 chr14 68874143 68979440 - ACTR3 chr2 113890063 113962596 + AHCY chr20 34280268 34311802 - AKAP13 chr15 85380571 85749358 + AL136454.1 chr1 192716132 192716653 + AL162417.1 chr9 133098121 133163914 - ALDH16A1 chr19 49453225 49471050 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-185-5p chr22 20033153 20033174 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-708-5p chr11 79402077 79402099 - hsa-miR-1294 chr5 154347153 154347174 + hsa-miR-1270 chr19 20399320 20399342 - hsa-miR-1276 chr15 85770548 85770567 - hsa-miR-3917 chr1 25906384 25906403 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC006064.5 chr12 6510275 6510522 + AC006460.2 chr2 190607660 190649840 - AC125807.2 chr12 3041437 3044950 + AC245033.4 chr15 82533175 82540008 - AL117378.1 chr6 131901963 131920565 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.