exRNAdisease

Entry Detail

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General Information

Database ID: exR0368619 RNA Name: RAN RNA Type: mRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 130872037 End Site(bp): 130877678 External Links: ENSG00000132341

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABT1 chr6 26596953 26600739 + AC004997.1 chr22 30285238 30299482 - AC006030.1 chr2 74211604 74363377 - AC010132.3 chr7 42909273 42932174 - AC092073.1 chr19 34396315 34409364 + AC124312.1 chr15 24955034 25000276 + AC135050.2 chr16 31083439 31094956 - ACAA2 chr18 49782164 49813953 - ACADVL chr17 7217125 7225266 + ACIN1 chr14 23058564 23095614 - ACO1 chr9 32384603 32454769 + ACO2 chr22 41469117 41528989 + ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR5 chr20 38748460 38772520 + ADAT3 chr19 1905399 1913447 + ADCY3 chr2 24819169 24919839 - ADGRG1 chr16 57610652 57665580 + ADH5 chr4 99070978 99088801 - ADSL chr22 40346500 40390463 + AEN chr15 88621337 88632281 + AFDN chr6 167826922 167972023 + AGO3 chr1 35930718 36072500 + AGPAT3 chr21 43865223 43987592 + AIF1L chr9 131096476 131123152 + AKAP1 chr17 57085092 57121346 + AKIRIN1 chr1 38991276 39006059 + AL021546.1 chr12 120438198 120460006 + AL022238.4 chr22 40346529 40410054 + AL136295.4 chr14 24180395 24190416 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-302b-3p chr4 112648489 112648511 - hsa-miR-302a-5p chr4 112648224 112648246 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC012236.1 chr15 31221999 31230838 - AC079781.5 chr7 97851688 97972985 - AC106864.1 chr4 112693047 112706810 - AC113189.4 chr17 7439506 7445966 + AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.