Entry Detail



General Information

Database ID:exR0368755
RNA Name:RCC2
RNA Type:mRNA
Chromosome:chr1
Starnd:-
Coordinate:
Start Site(bp):17406760End Site(bp):17439677
External Links:ENSG00000179051



Disease Information

Disease Name:Amyotrophic Lateral Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D000690
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis



Expression Detail

GEO ID:GSE121519
Description:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Experimental Design:Disease vs Control
Case Disease Type:Amyotrophic Lateral Sclerosis
Case Disease SubType:NA
Case Sample:Amyotrophic Lateral Sclerosis
Control Sample:Healthy
Number of Case:4
Number of Control:4
Number of Samples:8





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC002996.1
chr12
111753890
111791418
+
AC007192.1
chr19
18153158
18178117
+
AC010422.3
chr19
12643831
12648397
-
AC091167.2
chr15
90249556
90272208
+
AC097637.1
chr3
52221081
52231190
-
ACTA1
chr1
229431245
229434098
-
ACTB
chr7
5527148
5563784
-
ACTG1
chr17
81509971
81523847
-
ADCY5
chr3
123282296
123449758
-
ADD3
chr10
109996368
110135565
+
AL133352.1
chr10
100505628
100529881
-
AL136454.1
chr1
192716132
192716653
+
ALDH18A1
chr10
95605941
95656711
-
ALDH1B1
chr9
38392702
38398661
+
ALDH2
chr12
111766887
111817532
+
ALDOA
chr16
30064164
30070457
+
miRNA targets:
miRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa-miR-7-5p
chr19
4770700
4770723
+
hsa-miR-204-5p
chr9
70810031
70810052
-
hsa-miR-211-5p
chr15
31065095
31065116
-
hsa-miR-149-5p
chr2
240456015
240456037
+
hsa-miR-485-5p
chr14
101055427
101055448
+
hsa-miR-519c-5p
chr19
53686484
53686505
+
hsa-miR-620
chr12
116148575
116148594
-
hsa-miR-519b-5p
chr19
53695225
53695246
+
hsa-miR-1270
chr19
20399320
20399342
-
hsa-miR-4468
chr7
138123803
138123820
+
circRNA targets:NA
lncRNA targets:NA
Display:



Experiment Detail

GEO ID:GSE121519
Sample Source:Cerebrospinal Fluid
Source Fraction:Exosome
Platform:GPL18573
Method:NGS
Num of detected RNA Type:2
Num of detected RNAs of this Type:17787
Sample treatment protocol:Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant.
RNA Extract protocol:Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted.
RNA library preparation protocol:Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.



Reference

PMID:30630471
Title:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid
Author:Otake K, Kamiguchi H, Hirozane Y
Journal:BMC Med Genomics. 2019 Jan 10;12(1):7.
Description:The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.