exRNAdisease

Entry Detail

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General Information

Database ID: exR0368871 RNA Name: RHOA RNA Type: mRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 49359145 End Site(bp): 49412998 External Links: ENSG00000067560

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC012254.2 chr18 47108188 47176345 - AL603832.3 chr1 112702614 112711433 - AKAP9 chr7 91940867 92110673 + AC010422.3 chr19 12643831 12648397 - AC008982.1 chr19 38817471 38840178 - AC087289.3 chr17 75898645 75930129 - AL928654.3 chr14 105487199 105492267 + AC023055.1 chr12 55757275 55827546 - ABCE1 chr4 145098288 145129524 + ADIPOR1 chr1 202940826 202958572 - ADAM10 chr15 58588809 58749791 - ACAT2 chr6 159762045 159779112 + AL022238.4 chr22 40346529 40410054 + AC007192.1 chr19 18153158 18178117 + AHCY chr20 34280268 34311802 - AC008763.3 chr19 7678501 7682854 + ACTB chr7 5527148 5563784 - ADSL chr22 40346500 40390463 + ABHD14B chr3 51968510 51983409 - ACACA chr17 37084992 37406836 - ACIN1 chr14 23058564 23095614 - ACTG1 chr17 81509971 81523847 - AL590764.2 chrX 71103987 71111575 - AC135050.2 chr16 31083439 31094956 - ADNP2 chr18 80109262 80147523 + AC245033.1 chr15 82536788 82573194 - ACTL6A chr3 179562886 179588407 + AC027796.3 chr17 3585149 3636249 - ADCY1 chr7 45574140 45723116 + AK2 chr1 33007940 33080996 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-122-5p chr18 58451088 58451109 + hsa-miR-130b-3p chr22 21653354 21653375 + hsa-miR-454-3p chr17 59137787 59137809 - hsa-miR-1244 chr2 231713368 231713393 + hsa-miR-4284 chr7 73711327 73711344 + hsa-miR-4492 chr11 118910765 118910781 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005062.1 chr7 19918981 20140453 - AC007952.4 chr17 19112000 19112636 - AC023509.1 chr12 53441741 53467528 + AC073050.1 chr2 167293171 167558333 + AC093227.1 chr19 37728586 37730643 - AC245033.4 chr15 82533175 82540008 - AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.