exRNAdisease

Entry Detail

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General Information

Database ID: exR0369060 RNA Name: RPL18A RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 17859910 End Site(bp): 17864153 External Links: ENSG00000105640

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AL928654.3 chr14 105487199 105492267 + ALDOA chr16 30064164 30070457 + AC073508.2 chr17 40627356 40665141 - ADGRV1 chr5 90529344 91164437 + ACAA2 chr18 49782164 49813953 - AKT1 chr14 104769349 104795751 - AGPAT5 chr8 6708642 6761503 + ADD1 chr4 2843857 2930076 + ABCC10 chr6 43427366 43450427 + ACTB chr7 5527148 5563784 - AC010422.3 chr19 12643831 12648397 - ABHD5 chr3 43690108 43734371 + ADAM10 chr15 58588809 58749791 - ACTN4 chr19 38647649 38731589 + AASS chr7 122073549 122144255 - AGO2 chr8 140520156 140635633 - AC008982.1 chr19 38817471 38840178 - ACSF3 chr16 89088375 89164121 + AHCY chr20 34280268 34311802 - ACLY chr17 41866917 41930542 - AL359922.1 chr9 21802636 22029594 + AC104109.3 chr5 134167170 134226071 - AL358113.1 chr9 69035747 69255187 + ADAMTS10 chr19 8580240 8610735 - AC093525.2 chr16 2496032 2520218 + AC004922.1 chr7 99325879 99394653 + ABRAXAS2 chr10 124801819 124836667 + ACADVL chr17 7217125 7225266 + AL022318.4 chr22 39014363 39033276 + AC093525.1 chr16 2513965 2527955 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC245297.2 chr1 149264252 149264816 + AD000090.1 chr19 35557956 35581954 + AL021154.1 chr1 23549139 23550915 - AL117378.1 chr6 131901963 131920565 + AL137782.1 chr13 75549773 75807120 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.