exRNAdisease

Entry Detail

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General Information

Database ID: exR0369070 RNA Name: RPL27 RNA Type: mRNA Chromosome: chr17 Starnd: + Coordinate: Start Site(bp): 42998273 End Site(bp): 43002959 External Links: ENSG00000131469

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAAS chr12 53307456 53324864 - AAGAB chr15 67200667 67255195 - AARSD1 chr17 42950526 42964498 - AC010422.3 chr19 12643831 12648397 - AC018523.2 chr11 14493783 14520344 - AC025165.3 chr12 57772660 57797554 + AC092073.1 chr19 34396315 34409364 + AC093525.2 chr16 2496032 2520218 + AC138811.2 chr16 18788063 18801519 - AC245033.1 chr15 82536788 82573194 - ACADM chr1 75724347 75787575 + ACO1 chr9 32384603 32454769 + ACOT11 chr1 54542257 54639192 + ACSS1 chr20 25006230 25058980 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR2 chr2 65227753 65271253 + ACVR2B chr3 38453890 38493142 + ADM5 chr19 49688664 49690575 + AFTPH chr2 64524305 64593005 + AIF1L chr9 131096476 131123152 + AIMP1 chr4 106315544 106349456 + AKAP10 chr17 19904302 19978343 - AL121594.1 chr14 35122549 35317474 + AL358113.1 chr9 69035747 69255187 + AL365205.1 chr6 41780349 41790141 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-142-3p chr17 58331245 58331267 - hsa-miR-302d-3p chr4 112648006 112648028 - hsa-miR-4485-3p chr11 10508277 10508296 - hsa-miR-7705 chr8 100703002 100703024 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004687.1 chr17 58330884 58332508 - AC007952.4 chr17 19112000 19112636 - AC016027.1 chr22 18076527 18078884 - AC020663.1 chr16 4730115 4752565 - AC026362.1 chr12 122975320 122982907 + AC084082.1 chr8 66112667 66126632 + AC108134.2 chr16 3156736 3157483 - AC245033.4 chr15 82533175 82540008 - AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.