exRNAdisease

Entry Detail

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General Information

Database ID: exR0369071 RNA Name: RPL27A RNA Type: mRNA Chromosome: chr11 Starnd: + Coordinate: Start Site(bp): 8682788 End Site(bp): 8714759 External Links: ENSG00000166441

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC245033.1 chr15 82536788 82573194 - ABHD6 chr3 58237532 58295693 + ADGRE5 chr19 14380501 14408725 + ABCG2 chr4 88090150 88231628 - AL136454.1 chr1 192716132 192716653 + ABHD10 chr3 111979010 111993368 + ACTR3B chr7 152759749 152855378 + ACTN3 chr11 66546395 66563334 + ADCK2 chr7 140672945 140696261 + AGPAT3 chr21 43865223 43987592 + ALDOA chr16 30064164 30070457 + AKR1A1 chr1 45550543 45570049 + AHSA1 chr14 77457870 77469472 + AGGF1 chr5 77029251 77065234 + ACOT7 chr1 6264269 6394391 - AC068580.4 chr11 1734821 1763954 - AGBL2 chr11 47659591 47715389 - ADSL chr22 40346500 40390463 + AKR1B1 chr7 134442356 134459284 - AC007192.1 chr19 18153158 18178117 + AL022238.4 chr22 40346529 40410054 + ADGRL2 chr1 81306160 81992436 + ABCC4 chr13 95019835 95301475 - ACD chr16 67657512 67660815 - AIMP1 chr4 106315544 106349456 + AGPS chr2 177392757 177559299 + AC022826.2 chr8 73688691 73878854 - AC124312.1 chr15 24955034 25000276 + ACIN1 chr14 23058564 23095614 - AGA chr4 177430774 177442437 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-3064-5p chr17 64500817 64500837 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004687.1 chr17 58330884 58332508 - AC016876.2 chr17 7581964 7584086 - AC020978.5 chr16 68225969 68229145 - AC023509.1 chr12 53441741 53467528 + AC068768.1 chr12 123262060 123262402 + AC074183.1 chr7 84939349 84940245 - AC090114.2 chr7 128524016 128531069 - AC090825.1 chr15 99805793 99916818 + AC106864.1 chr4 112693047 112706810 - AC132217.1 chr11 2129121 2129964 - AC245014.3 chr1 145281116 145281462 + AC245033.4 chr15 82533175 82540008 - AL021707.2 chr22 38667585 38681847 - AL022311.1 chr22 37876148 37895563 + AL031595.3 chr22 44139365 44153626 + AL035458.2 chr20 34234840 34281173 - AL050341.2 chr1 40256427 40257967 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.