exRNAdisease

Entry Detail

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General Information

Database ID: exR0369072 RNA Name: RPL28 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 55385345 End Site(bp): 55403250 External Links: ENSG00000108107

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCD3 chr1 94418389 94518666 + AC004832.3 chr22 30409255 30428990 + AC004922.1 chr7 99325879 99394653 + AC005258.1 chr19 2269525 2341172 + AC006064.6 chr12 6556886 6607367 - AC010323.1 chr19 8308283 8321379 - AC010422.3 chr19 12643831 12648397 - AC024592.3 chr19 5866171 5903787 - AC073508.2 chr17 40627356 40665141 - AC093525.2 chr16 2496032 2520218 + AC104389.5 chr11 5254392 5678434 - AC138811.2 chr16 18788063 18801519 - ACAT2 chr6 159762045 159779112 + ACLY chr17 41866917 41930542 - ACO2 chr22 41469117 41528989 + ACTA1 chr1 229431245 229434098 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ADGRL1 chr19 14147743 14206187 - AEBP2 chr12 19404045 19720801 + AGRN chr1 1020120 1056118 + AHCY chr20 34280268 34311802 - AKAP13 chr15 85380571 85749358 + AL049629.2 chr11 33698261 33722467 - AL669918.1 chr6 32813767 32838822 - ALAS1 chr3 52198086 52214327 + ALDH2 chr12 111766887 111817532 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-6805-5p chr19 55388186 55388207 + hsa-miR-6805-3p chr19 55388220 55388242 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007952.4 chr17 19112000 19112636 - AC010327.5 chr19 55216660 55221616 + AC012360.3 chr2 105334027 105337475 - AC016747.1 chr2 61141592 61144969 - AC020928.2 chr19 36773712 36775908 - AC026362.1 chr12 122975320 122982907 + AC026471.1 chr16 31456711 31459736 - AC091564.7 chr11 6610883 6616594 - AC139149.1 chr17 81514047 81527776 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.