exRNAdisease

Entry Detail

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General Information

Database ID: exR0369075 RNA Name: RPL30 RNA Type: mRNA Chromosome: chr8 Starnd: - Coordinate: Start Site(bp): 98024851 End Site(bp): 98046469 External Links: ENSG00000156482

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004922.1 chr7 99325879 99394653 + AC010422.3 chr19 12643831 12648397 - AC069503.2 chr12 121888809 121921470 + AC073508.2 chr17 40627356 40665141 - AC090517.4 chr15 56652488 56918571 - AC120057.2 chr17 7240427 7244635 - AC245033.1 chr15 82536788 82573194 - ACADM chr1 75724347 75787575 + ACAT2 chr6 159762045 159779112 + ACSL3 chr2 222860942 222944639 + ACTB chr7 5527148 5563784 - ACTR1A chr10 102461881 102502712 - ADSL chr22 40346500 40390463 + AGTRAP chr1 11736084 11754802 + AHCY chr20 34280268 34311802 - AICDA chr12 8602170 8612867 - AL022238.4 chr22 40346529 40410054 + AL136295.5 chr14 24147548 24166452 + AL138752.2 chr9 37588413 38068687 - AL513165.2 chr9 37512547 37592469 - AL713999.1 chr1 155169409 155187272 - ALDH18A1 chr10 95605941 95656711 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC027097.1 chr18 57630302 57669296 + AC027097.2 chr18 57639455 57738603 + AC105339.2 chr15 82752884 82757208 + AC245033.4 chr15 82533175 82540008 - AL132780.1 chr14 22929607 22956374 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.