exRNAdisease

Entry Detail

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General Information

Database ID: exR0369077 RNA Name: RPL32 RNA Type: mRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 12834485 End Site(bp): 12841582 External Links: ENSG00000144713

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCB10 chr1 229516582 229558707 - ABHD4 chr14 22598237 22613215 + AC004805.1 chr17 65538102 65641033 - AC010132.3 chr7 42909273 42932174 - AC023055.1 chr12 55757275 55827546 - AC073896.1 chr12 56285916 56316059 - AC104109.3 chr5 134167170 134226071 - ACO2 chr22 41469117 41528989 + ACTB chr7 5527148 5563784 - ACTR2 chr2 65227753 65271253 + ADAM10 chr15 58588809 58749791 - ADD3 chr10 109996368 110135565 + ADNP chr20 50888916 50931437 - AFTPH chr2 64524305 64593005 + AGO1 chr1 35869808 35930532 + AHCY chr20 34280268 34311802 - AK3 chr9 4709556 4742043 - AL645941.2 chr6 32937364 32953122 - ALCAM chr3 105366909 105576900 + ALDOA chr16 30064164 30070457 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007218.1 chr16 9666885 9676843 - AC019294.2 chr15 75737820 75763322 + AC021078.1 chr5 149494314 149504670 - AC026362.1 chr12 122975320 122982907 + AC087190.3 chr16 9104848 9113181 + AC087280.2 chr11 6821942 6926878 - AC117386.2 chr3 150039214 150213726 + AC124068.2 chr15 89335053 89336161 + AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.