exRNAdisease

Entry Detail

---



General Information

Database ID: exR0369089 RNA Name: RPL4 RNA Type: mRNA Chromosome: chr15 Starnd: - Coordinate: Start Site(bp): 66498015 End Site(bp): 66524532 External Links: ENSG00000174444

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AIF1L chr9 131096476 131123152 + ADD1 chr4 2843857 2930076 + AL049629.2 chr11 33698261 33722467 - AL133352.1 chr10 100505628 100529881 - AASDHPPT chr11 106075501 106098699 + AC004223.3 chr17 35011349 35121493 - ABT1 chr6 26596953 26600739 + ADSL chr22 40346500 40390463 + AC245033.1 chr15 82536788 82573194 - AC013394.1 chr15 92883413 92949230 + AC008982.1 chr19 38817471 38840178 - AC003002.3 chr19 57389884 57419994 + ACTN4 chr19 38647649 38731589 + ADD2 chr2 70607618 70768225 - ADGRL2 chr1 81306160 81992436 + AC004922.1 chr7 99325879 99394653 + AC120057.2 chr17 7240427 7244635 - ALAD chr9 113386312 113401290 - ACTC1 chr15 34790230 34795549 - ADAR chr1 154582057 154628013 - AIMP1 chr4 106315544 106349456 + ADCY3 chr2 24819169 24919839 - AC015813.2 chr17 57989038 58007246 - ABRACL chr6 139028745 139043302 + AD000671.2 chr19 35739252 35745432 - ACP5 chr19 11574660 11579008 - AGBL5 chr2 27042364 27070622 + ABCC4 chr13 95019835 95301475 - AC007192.1 chr19 18153158 18178117 + AGAP2 chr12 57723761 57742157 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-3654 chr7 133034860 133034878 - hsa-miR-4745-5p chr19 804941 804963 + hsa-miR-6889-5p chr22 41253023 41253045 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005899.4 chr17 32328441 32329395 + AC007952.4 chr17 19112000 19112636 - AC016026.1 chr22 17787652 17811497 - AC016876.2 chr17 7581964 7584086 - AC026362.1 chr12 122975320 122982907 + AC051619.7 chr15 45200325 45200632 - AC126365.1 chr17 20788071 20789584 + AC245033.4 chr15 82533175 82540008 - AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + AL117378.1 chr6 131901963 131920565 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.