exRNAdisease

Entry Detail

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General Information

Database ID: exR0369091 RNA Name: RPL5 RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 92832013 End Site(bp): 92841924 External Links: ENSG00000122406

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCB4 chr7 87401697 87480435 - AC004922.1 chr7 99325879 99394653 + AGTPBP1 chr9 85546539 85742029 - ACO1 chr9 32384603 32454769 + AL136454.1 chr1 192716132 192716653 + ALDH18A1 chr10 95605941 95656711 - ACTR3 chr2 113890063 113962596 + ADSL chr22 40346500 40390463 + AC073896.1 chr12 56285916 56316059 - AC015802.6 chr17 76563710 76570544 - AC091167.2 chr15 90249556 90272208 + AGK chr7 141551278 141655244 + ACIN1 chr14 23058564 23095614 - AGO1 chr1 35869808 35930532 + AASS chr7 122073549 122144255 - ABL1 chr9 130713016 130887675 + AL022238.4 chr22 40346529 40410054 + AL365273.2 chr10 95860548 95924844 + ACP6 chr1 147629652 147670524 - ACVR1B chr12 51951699 51997078 + ALDOA chr16 30064164 30070457 + ACTR2 chr2 65227753 65271253 + AGA chr4 177430774 177442437 - AK2 chr1 33007940 33080996 - AEN chr15 88621337 88632281 + ADNP2 chr18 80109262 80147523 + AC073612.1 chr12 71674204 71698968 + AL121594.1 chr14 35122549 35317474 + ACTB chr7 5527148 5563784 - AGRN chr1 1020120 1056118 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-3654 chr7 133034860 133034878 - hsa-miR-4426 chr1 192716333 192716349 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010163.1 chr10 78293842 78301003 + AC018521.1 chr17 47945424 47981736 + AC022167.2 chr16 8848105 8860456 + AC024940.6 chr12 31280422 31280895 - AC104461.1 chr1 200333193 200478669 + AC132217.1 chr11 2129121 2129964 - AC139149.1 chr17 81514047 81527776 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.