exRNAdisease

Entry Detail

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General Information

Database ID: exR0369132 RNA Name: RPS27A RNA Type: mRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 55231903 End Site(bp): 55235853 External Links: ENSG00000143947

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCF2 chr7 151207837 151227166 - ABHD2 chr15 89087459 89202355 + ABRACL chr6 139028745 139043302 + AC068946.1 chr2 219075329 219170827 - AC091167.2 chr15 90249556 90272208 + AC245033.1 chr15 82536788 82573194 - ACAD8 chr11 134253495 134265855 + ACLY chr17 41866917 41930542 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR2 chr2 65227753 65271253 + ACVR1 chr2 157736444 157876330 - ADAR chr1 154582057 154628013 - ADD1 chr4 2843857 2930076 + ADORA2A chr22 24417879 24442357 + ADSL chr22 40346500 40390463 + AGAP1 chr2 235494043 236131800 + AGPAT4 chr6 161129967 161274061 - AGTPBP1 chr9 85546539 85742029 - AHNAK chr11 62433542 62556235 - AIF1L chr9 131096476 131123152 + AL022238.4 chr22 40346529 40410054 + AL133352.1 chr10 100505628 100529881 - AL136454.1 chr1 192716132 192716653 + AL139300.1 chr14 103562962 103685924 + AL355315.1 chr10 97584374 97673910 + ALDH7A1 chr5 126531200 126595362 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4426 chr1 192716333 192716349 + hsa-miR-4485-3p chr11 10508277 10508296 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AC091564.7 chr11 6610883 6616594 - AC104257.1 chr8 131308545 131317632 + AC106864.1 chr4 112693047 112706810 - AC239868.1 chr1 149861271 149862504 + AC245033.4 chr15 82533175 82540008 - AL031656.1 chr20 42685404 42688562 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.