exRNAdisease

Entry Detail

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General Information

Database ID: exR0369140 RNA Name: RPS5 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 58386400 End Site(bp): 58394806 External Links: ENSG00000083845

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AGFG2 chr7 100539203 100568220 + AL358113.1 chr9 69035747 69255187 + AKAP1 chr17 57085092 57121346 + ACO2 chr22 41469117 41528989 + AL022238.4 chr22 40346529 40410054 + AC004832.3 chr22 30409255 30428990 + AC007192.1 chr19 18153158 18178117 + AC139530.2 chr17 81703371 81720539 + AC010422.3 chr19 12643831 12648397 - AC092073.1 chr19 34396315 34409364 + AC040162.1 chr16 67929614 67936017 - ABRACL chr6 139028745 139043302 + ACTN4 chr19 38647649 38731589 + AHDC1 chr1 27534035 27604431 - AHCYL1 chr1 109984765 110023742 + AHCY chr20 34280268 34311802 - ACTR1B chr2 97655939 97664044 - ACTB chr7 5527148 5563784 - ALDOA chr16 30064164 30070457 + AK2 chr1 33007940 33080996 - AC008763.3 chr19 7678501 7682854 + AC002985.1 chr19 18907013 18934397 - AC011462.1 chr19 41350853 41425001 + ABHD10 chr3 111979010 111993368 + AC018523.2 chr11 14493783 14520344 - AC104452.1 chr3 49416777 49429314 - AHSA1 chr14 77457870 77469472 + ABCC1 chr16 15949577 16143074 + ADSL chr22 40346500 40390463 + AACS chr12 125065434 125143333 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1244 chr2 231713368 231713393 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC026362.1 chr12 122975320 122982907 + AC091564.7 chr11 6610883 6616594 - AC121761.1 chr12 75483454 75489820 - AD000090.1 chr19 35557956 35581954 + AL121832.3 chr20 62402236 62405935 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.