exRNAdisease

Entry Detail

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General Information

Database ID: exR0369155 RNA Name: RPSA RNA Type: mRNA Chromosome: chr3 Starnd: + Coordinate: Start Site(bp): 39406716 End Site(bp): 39412542 External Links: ENSG00000168028

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABHD12 chr20 25294742 25390835 - ABHD2 chr15 89087459 89202355 + AC008575.1 chr5 112827213 112867582 + AC018523.2 chr11 14493783 14520344 - AC104109.3 chr5 134167170 134226071 - ACACA chr17 37084992 37406836 - ACSL6 chr5 131949973 132012243 - ACTA2 chr10 88935074 88991339 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - AFF4 chr5 132875395 132963634 - AGFG1 chr2 227472152 227561214 + AHCY chr20 34280268 34311802 - AL355987.3 chr9 136791379 136800595 + ALDH7A1 chr5 126531200 126595362 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004687.1 chr17 58330884 58332508 - AC005618.1 chr5 141326210 141329357 + AC005899.4 chr17 32328441 32329395 + AC006064.5 chr12 6510275 6510522 + AC016876.2 chr17 7581964 7584086 - AC019205.1 chr6 73263212 73301789 + AC023509.1 chr12 53441741 53467528 + AC068205.2 chr11 43578889 43840030 + AC087190.3 chr16 9104848 9113181 + AC091564.7 chr11 6610883 6616594 - AC124312.3 chr15 25087661 25088896 + AC132217.1 chr11 2129121 2129964 - AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.