Entry Detail



General Information

Database ID:exR0370036
RNA Name:SMARCD1
RNA Type:mRNA
Chromosome:chr12
Starnd:+
Coordinate:
Start Site(bp):50085200End Site(bp):50100707
External Links:ENSG00000066117



Disease Information

Disease Name:Amyotrophic Lateral Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D000690
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis



Expression Detail

GEO ID:GSE121519
Description:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Experimental Design:Disease vs Control
Case Disease Type:Amyotrophic Lateral Sclerosis
Case Disease SubType:NA
Case Sample:Amyotrophic Lateral Sclerosis
Control Sample:Healthy
Number of Case:4
Number of Control:4
Number of Samples:8





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ABHD14B
chr3
51968510
51983409
-
AC005258.1
chr19
2269525
2341172
+
AC008763.2
chr19
7629796
7643048
+
AC008982.1
chr19
38817471
38840178
-
AC099811.2
chr17
42119674
42154916
-
ADCY6
chr12
48766194
48789037
-
AGTPBP1
chr9
85546539
85742029
-
AIF1L
chr9
131096476
131123152
+
AL139300.1
chr14
103562962
103685924
+
AL589666.1
chr6
85557978
85615234
-
ALDOA
chr16
30064164
30070457
+
miRNA targets:
miRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa-miR-708-5p
chr11
79402077
79402099
-
hsa-miR-1286
chr22
20249145
20249165
-
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016876.2
chr17
7581964
7584086
-
AC245014.3
chr1
145281116
145281462
+
AD000090.1
chr19
35557956
35581954
+
Display:



Experiment Detail

GEO ID:GSE121519
Sample Source:Cerebrospinal Fluid
Source Fraction:Exosome
Platform:GPL18573
Method:NGS
Num of detected RNA Type:2
Num of detected RNAs of this Type:17787
Sample treatment protocol:Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant.
RNA Extract protocol:Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted.
RNA library preparation protocol:Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.



Reference

PMID:30630471
Title:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid
Author:Otake K, Kamiguchi H, Hirozane Y
Journal:BMC Med Genomics. 2019 Jan 10;12(1):7.
Description:The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.