Entry Detail



General Information

Database ID:exR0370328
RNA Name:SPTAN1
RNA Type:mRNA
Chromosome:chr9
Starnd:+
Coordinate:
Start Site(bp):128552558End Site(bp):128633662
External Links:ENSG00000197694



Disease Information

Disease Name:Amyotrophic Lateral Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D000690
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis



Expression Detail

GEO ID:GSE121519
Description:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Experimental Design:Disease vs Control
Case Disease Type:Amyotrophic Lateral Sclerosis
Case Disease SubType:NA
Case Sample:Amyotrophic Lateral Sclerosis
Control Sample:Healthy
Number of Case:4
Number of Control:4
Number of Samples:8





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACKR3
chr2
236567787
236582354
+
ACTB
chr7
5527148
5563784
-
ADCY1
chr7
45574140
45723116
+
ADCY9
chr16
3953387
4116442
-
ADK
chr10
74151202
74709963
+
AGPAT3
chr21
43865223
43987592
+
AKR1A1
chr1
45550543
45570049
+
AKT2
chr19
40230317
40285536
-
miRNA targets:
miRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa-miR-185-5p
chr22
20033153
20033174
+
hsa-miR-654-5p
chr14
101040234
101040255
+
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC002116.2
chr19
36014508
36045972
+
AC069281.2
chr7
100572232
100578700
-
AL136379.1
chr1
228119149
228121312
-
Display:



Experiment Detail

GEO ID:GSE121519
Sample Source:Cerebrospinal Fluid
Source Fraction:Exosome
Platform:GPL18573
Method:NGS
Num of detected RNA Type:2
Num of detected RNAs of this Type:17787
Sample treatment protocol:Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant.
RNA Extract protocol:Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted.
RNA library preparation protocol:Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.



Reference

PMID:30630471
Title:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid
Author:Otake K, Kamiguchi H, Hirozane Y
Journal:BMC Med Genomics. 2019 Jan 10;12(1):7.
Description:The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.