Entry Detail



General Information

Database ID:exR0371325
RNA Name:TNRC6B
RNA Type:mRNA
Chromosome:chr22
Starnd:+
Coordinate:
Start Site(bp):40044817End Site(bp):40335808
External Links:ENSG00000100354



Disease Information

Disease Name:Amyotrophic Lateral Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D000690
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis



Expression Detail

GEO ID:GSE121519
Description:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Experimental Design:Disease vs Control
Case Disease Type:Amyotrophic Lateral Sclerosis
Case Disease SubType:NA
Case Sample:Amyotrophic Lateral Sclerosis
Control Sample:Healthy
Number of Case:4
Number of Control:4
Number of Samples:8





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC009133.6
chr16
29812261
29820092
+
ADCY6
chr12
48766194
48789037
-
AHI1
chr6
135283532
135497765
-
miRNA targets:
miRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa-let-7a-5p
chr9
94175962
94175983
+
hsa-let-7b-5p
chr22
46113691
46113712
+
hsa-let-7e-5p
chr19
51692793
51692814
+
hsa-miR-129-5p
chr7
128207876
128207896
+
hsa-miR-147a
chr9
120244985
120245004
-
hsa-miR-34a-5p
chr1
9151735
9151756
-
hsa-let-7g-5p
chr3
52268336
52268357
-
hsa-let-7i-5p
chr12
62603691
62603712
+
hsa-miR-140-5p
chr16
69933103
69933124
+
hsa-miR-142-3p
chr17
58331245
58331267
-
hsa-miR-185-5p
chr22
20033153
20033174
+
hsa-miR-206
chr6
52144401
52144422
+
hsa-miR-200a-3p
chr1
1167916
1167937
+
hsa-miR-34c-5p
chr11
111513451
111513473
+
hsa-miR-449a
chr5
55170586
55170607
-
hsa-miR-613
chr12
12764709
12764728
+
hsa-miR-449b-5p
chr5
55170706
55170727
-
hsa-miR-501-3p
chrX
50009772
50009793
+
hsa-miR-502-3p
chrX
50014649
50014670
+
hsa-miR-616-3p
chr12
57519183
57519204
-
hsa-miR-1286
chr22
20249145
20249165
-
hsa-miR-1321
chrX
85835832
85835849
+
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004687.1
chr17
58330884
58332508
-
Display:



Experiment Detail

GEO ID:GSE121519
Sample Source:Cerebrospinal Fluid
Source Fraction:Exosome
Platform:GPL18573
Method:NGS
Num of detected RNA Type:2
Num of detected RNAs of this Type:17787
Sample treatment protocol:Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant.
RNA Extract protocol:Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted.
RNA library preparation protocol:Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.



Reference

PMID:30630471
Title:Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid
Author:Otake K, Kamiguchi H, Hirozane Y
Journal:BMC Med Genomics. 2019 Jan 10;12(1):7.
Description:The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.