exRNAdisease

Entry Detail

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General Information

Database ID: exR0371388 RNA Name: TPM4 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 16067021 End Site(bp): 16103002 External Links: ENSG00000167460

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAMDC chr11 77821109 77918432 + AAMP chr2 218264123 218270257 - AARSD1 chr17 42950526 42964498 - ABL1 chr9 130713016 130887675 + AC004922.1 chr7 99325879 99394653 + AC007192.1 chr19 18153158 18178117 + AC048338.1 chr12 122207779 122266423 - AC138696.1 chr8 143247110 143276403 + AC245033.1 chr15 82536788 82573194 - ACLY chr17 41866917 41930542 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ADCY5 chr3 123282296 123449758 - ADCY6 chr12 48766194 48789037 - ADIPOR2 chr12 1688574 1788674 + ADPGK chr15 72751369 72785846 - ADRM1 chr20 62302093 62308862 + AGAP3 chr7 151085831 151144436 + AHCY chr20 34280268 34311802 - AKT2 chr19 40230317 40285536 - AL136454.1 chr1 192716132 192716653 + AL365205.1 chr6 41780349 41790141 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-139-5p chr11 72615102 72615124 - hsa-miR-185-5p chr22 20033153 20033174 + hsa-miR-1270 chr19 20399320 20399342 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC008969.1 chr19 58002061 58011232 + AC016876.2 chr17 7581964 7584086 - AC026362.1 chr12 122975320 122982907 + AC026464.2 chr16 69463844 69466264 + AC079807.1 chr2 47905678 47907810 + AC099343.3 chr4 184471924 184472609 - AC132217.1 chr11 2129121 2129964 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.