exRNAdisease

Entry Detail

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General Information

Database ID: exR0373020 RNA Name: DSCAM-AS1 RNA Type: lncRNA Chromosome: chr21 Starnd: + Coordinate: Start Site(bp): 40383083 End Site(bp): 40385358 External Links: ENSG00000235123

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: NA miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-509-3p chrX 147258775 147258796 - hsa-miR-144-5p chr17 28861583 28861604 - hsa-miR-3163 chr11 66934476 66934497 - hsa-miR-3174 chr15 90006764 90006786 + hsa-miR-433-3p chr14 100881949 100881970 + hsa-miR-4784 chr2 131491208 131491227 - hsa-miR-513b-5p chrX 147199093 147199114 - hsa-miR-494-3p chr14 101029681 101029702 + hsa-miR-580-3p chr5 36147907 36147928 - hsa-miR-3150a-3p chr8 95072962 95072983 + hsa-miR-186-5p chr1 71067681 71067702 - hsa-miR-3150b-3p chr8 95072924 95072944 - hsa-miR-3612 chr12 128294105 128294126 + hsa-miR-204-5p chr9 70810031 70810052 - hsa-miR-122-5p chr18 58451088 58451109 + hsa-miR-380-3p chr14 101025056 101025077 + hsa-miR-374a-3p chrX 74287295 74287316 - hsa-miR-299-3p chr14 101023832 101023853 + hsa-miR-136-5p chr14 100884716 100884738 + hsa-miR-2355-3p chr2 207109999 207110020 - hsa-miR-6849-5p chr8 144400318 144400340 - hsa-miR-877-5p chr6 30584332 30584351 + hsa-miR-3611 chr10 35079609 35079629 - hsa-miR-577 chr4 114656774 114656794 + hsa-miR-5094 chr15 89850690 89850711 - hsa-miR-6763-5p chr12 132582002 132582020 + hsa-miR-650 chr22 22822791 22822811 + hsa-miR-6875-5p chr7 100868041 100868061 + hsa-miR-2681-3p chr13 101967665 101967686 - hsa-miR-147a chr9 120244985 120245004 - circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.