exRNAdisease

Entry Detail

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General Information

Database ID: exR0373027 RNA Name: EPB41L4A-AS1 RNA Type: lncRNA Chromosome: chr5 Starnd: + Coordinate: Start Site(bp): 112160526 End Site(bp): 112164818 External Links: ENSG00000224032

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007192.1 chr19 18153158 18178117 + AC120057.2 chr17 7240427 7244635 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - AGRN chr1 1020120 1056118 + AC118553.2 chr1 99970011 100083321 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-17-5p chr13 91350618 91350640 + hsa-miR-20a-5p chr13 91351072 91351094 + hsa-miR-93-5p chr7 100093815 100093837 - hsa-miR-105-5p chrX 152394458 152394480 - hsa-miR-106a-5p chrX 134170244 134170266 - hsa-miR-214-3p chr1 172138816 172138837 - hsa-miR-142-5p chr17 58331283 58331303 - hsa-miR-106b-5p chr7 100094043 100094063 - hsa-miR-20b-5p chrX 134169850 134169872 - hsa-miR-519c-3p chr19 53686522 53686543 + hsa-miR-526b-3p chr19 53694443 53694464 + hsa-miR-519b-3p chr19 53695263 53695284 + hsa-miR-519d-3p chr19 53713400 53713421 + hsa-miR-520g-3p chr19 53722220 53722243 + hsa-miR-520h chr19 53742566 53742587 + hsa-miR-519a-3p chr19 53762397 53762418 + hsa-miR-761 chr1 51836375 51836396 - hsa-miR-3619-5p chr22 46091059 46091080 + hsa-miR-5590-3p chr2 134857853 134857873 + hsa-miR-1277-5p chrX 118386401 118386424 + hsa-miR-511-3p chr10 17845160 17845179 + hsa-miR-7853-5p chr6 6169380 6169400 - circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.